Journal of Clinical and Translational Hepatology

Journal of Clinical and Translational Hepatology

Thursday, 12 / 09 / 2021

Articles

Abstract

ORIGINAL ARTICLE

Potential Role and Clinical Value of PPP2CA in Hepatocellular Carcinoma

Cheng-Lei Yang1,#, Xue Qiu2,#, Jin-Yan Lin2, Xiao-Yu Chen3, Yu-Mei Zhang4, Xiao-Yin Hu1, Jian-Hong Zhong1, Shen Tang5, Xi-Yi Li6, Bang-De Xiang1,* and Zhi-Ming Zhang1,*

1  Department of Hepatobiliary Surgery, Guangxi Medical University Cancer Hospital, Nanning, Guangxi, China
2  The First Clinical Medical School, Guangxi Medical University, Nanning, Guangxi, China
3  Department of Pathology, Guangxi Medical University Cancer Hospital, Nanning, Guangxi, China
4  Department of Medical Oncology, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi, China
5  School of Basic Medical Sciences, Guangxi Medical University, Nanning, Guangxi, China
6  School of Public Health, Guangxi Medical University, Nanning, Guangxi, China
#Both authors contributed equally to this work.
*Correspondence to:Zhi-Ming Zhang and Bang-De Xiang, Hepatobiliary Surgery Department, Guangxi Liver Cancer Diagnosis and Treatment Engineering and Technology Research Center, Guangxi Medical University Cancer Hospital, He Di Rd #71, Nanning, Guangxi 530021, China. ORCID:https://orcid.org/0000-0001-9823-4945(ZMZ), https://orcid.org/0000-0002-1877-7139(BDX). Tel: +86-771-533-0855, Fax: +86-771-531-2000, E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. (ZMZ),This email address is being protected from spambots. You need JavaScript enabled to view it. (BDX).

Journal of Clinical and Translational Hepatology 2021;9(5):661-671 DOI: 10.14218/JCTH.2020.00168
Received:December 16, 2020 Accepted:April 5, 2021 Published online:May 13, 2021

Abstract

Background and Aims:Protein phosphatase 2A (PP2A) is associated with many cancers. This study aimed to clarify whether PPP2CA, which encodes the alpha isoform of the catalytic subunit of PP2A, plays a role in hepatocellular carcinoma (HCC) and to identify the potential underlying molecular pathways.

Methods:Based on bioinformatics, public databases and our in-house RNA-Seq database, we analyzed the clinical value and molecular mechanism of PPP2CA in HCC.

Results:Data were analyzed from 2,545 patients with HCC and 1,993 controls without HCC indexed in The Cancer Genome Atlas database, the Gene Expression Omnibus database and our in-house RNA-Seq database. PPP2CA expression was significantly higher in HCC tissue than in non-cancerous tissues (standardized mean difference: 0.69, 95% confidence interval [CI]: 0.50–0.89). PPP2CA expression was able to differentiate HCC from non-HCC, with an area under the summary receiver operator characteristic curve of 0.79 (95% CI: 0.75–0.83). Immunohistochemistry of tissue sections confirmed that PPP2CA protein was up-regulated in HCC tissues. High PPP2CA expression in HCC patients was associated with shorter overall, progression-free and disease-free survival. Potential molecular pathways through which PPP2CA may be involved in HCC were determined using miRWalk 2.0 as well as analysis of Gene Ontology categories, Kyoto Encyclopedia of Genes and Genomes pathways, and protein-protein interaction networks.

Conclusions:PPP2CA is up-regulated in HCC and higher expression correlates with worse prognosis. PPP2CA shows potential as a diagnostic marker for HCC. Future studies should examine whether PPP2CA contributes to HCC through the candidate microRNAs, pathways and hub genes identified in this study.

Keywords

Hepatocellular carcinoma, PPP2CA, Molecular mechanism, Diagnostic marker

Journal of Clinical and Translational Hepatology 2021 vol. 9, 661-671  [ Html  ] [ PDF Full-text ]

© 2021 Authors. This is an Open Access article distributed under the terms of the  Creative Commons Attribution-Noncommercial 4.0 License(CC BY-NC 4.0), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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